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KMID : 1101420190510030316
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Korean Journal of Clinical Laboratory Science
2019 Volume.51 No. 3 p.316 ~ p.322
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Effect of Evodiae fructus Methanol Extract on Virulence-Related Genes’ Expression of Helicobacter pylori
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Yang Ji-Yeong
Lee Pyeong-Jae
Kim Jong-Bae
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Abstract
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Helicobacter pylori, a gram-negative bacterium, is one of the risk factors that induces gastritis and gastric cancer. Therefore, much attention has been paid to the compounds that inhibit bacterial growth or eradicate bacteria. Evodiae fructus (EF), the fruit of Evodia rutaecarpa, has been used for treating diarrhea and abdominal pain. EF extract was already found to inhibit the growth of H. pylori.
However, to the best of our knowledge, the effect of EF on the virulence factors of H. pylori has not been reported. In this study, when comparing the minimum inhibitory concentration (MIC) of the different methanol concentration extracts, the 95% methanol extract (EF95) showed the lowest MIC value. EF95 extract suppressed the expressions of cagA, vacA and ureB, but interestingly, it up-regulated the expression of ureA. A decrease in production of ammonia in the culture medium and the cell lysates indicated that EF95 inhibited the urease activity in H. pylori, which was the result of EF95 inhibiting the ureB expression. Although the mechanism by which EF95 extract regulates the virulence factors in H. pylori needs further study, EF95 could be used for treatment of gastric troubles induced by H. pylori.
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KEYWORD
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CagA, Evodiae fructus, Helicobacter pylori, Urease, VacA
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